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#21
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Newbie question about macro with DSLR
Eric Smith wrote:
Paul Furman writes: On the other hand, how much detail do you need? I'd like to resolve details with a minimum feature size of 3 microns. Defininately use a microscope with a camera adapter. jue |
#22
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Newbie question about macro with DSLR
Jürgen Exner wrote:
Eric Smith wrote: Paul Furman writes: On the other hand, how much detail do you need? I'd like to resolve details with a minimum feature size of 3 microns. Defininately use a microscope with a camera adapter. jue He does NOT need a microscope ... only a microscope objective and some way to focus it. As to focusing a microscope objective: the manufacturer specifies the back focus ... that is, the distance from the mounting screw to were the focal plane of the camera is. You just make sure that that distance is about right, like within 5 mm. Then you focus by moving the subject, or by small movement of the objective. Doug McDonald |
#23
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Newbie question about macro with DSLR
On Sun, 27 Apr 2008 16:54:31 -0700, Eric Smith
wrote: I'm considering buying a Canon Digital Rebel XSi for a project involving macro photography, and I'm looking for advice regarding macro lenses, reverse mounts, etc. Basically I'm trying to photograph a flat subject such that an area about 1 mm on a side will be full-frame. It would be better yet if I could do that for an area 0.5 mm on a side. The camera has an APS-C size sensor (22.2 x 14.8 mm). I have a precision X-Y stage with stepper motors and microstepping control, so I can position the subject in increments of less than 0.1 mm. I plan to take many photos with a small shift, and stitch them together, probably using Hugin. Because I expect there to be a lot of overlap between the images, I think I can tolerate some amount of vignetting, spherical abberation, etc. Is this feasible with some macro lens, combination of lenses, reversing ring, extension tube, etc? I'm hoping to avoid the need to buy a microscope and adapter, due to the cost, though that's obviously an option. I'm not sure how to tell whether a particular microscope would be suitable without trying it; for instance, I've looked at a 7x-90x microscope, but apparently 10x or 20x of that magnification is due to the magnification of the eyepiece, which I wouldn't have with the camera adapter. If I understand correctly, you need a magnification of around 10 to 20. By this I mean that the image size on the sensor of the camera is 10 to 20 times as big as what you are shooting. I agree with some other posters that your best solution might be a microscope. You should also be able to achieve this by reversing an ordinary Canon lens. A little background first... Lenses are designed to work best at particular subject and lens extension distances... normally the optimal subject distance is from infinity to around 10 times the focal length, and this corresponds to a lens to sensor distance of approximately the focal length plus a couple of millimeters. To use an example, consider a "normal lens", which would be about 35mm focal length for the Excess Eye (XSi). Depending in which lens exactly, it will work great focused from infinity to about 300 mm from the lens. If you try to get closer by using extension rings, you will be able to focus closer, but the optical quality starts to deteriorate a bit. When you get so close that you are near 1:1 (image and subject about the same size), image quality is worse, and continues to get worse as you go greater than 1. To some extent you can manage by stopping down, but that is of limited use. The fix is to reverse the lens when you need to shoot well over 1:1. Then the lens becomes happy again because its distances once again are close to its ideal distances... except that everything is in reverse. Thus the distance from the back of the lens to the subject is now about the same as lens-sensor when shooting at normal distances, and the distance from the front of the lens to the sensor is now around 300 mm or greater, which is about right for this lens. The light rays don't mind going backwards. To give some examples --- You are shooting a pair of shoes or whatever with the lens mounted normally. The distance to the subject is 400 mm. With that distance, the magnification on the sensor will be m = 0.1, and the lens extension is 3.4mm... so the distance from the center of the lens to the sensor is 35 + 3.4 = 38.4 mm. Now you reverse the lens using a reverse adapter, and shoot something very tiny. The lens-subject distance is very close at 38mm, the lens-sensor is racked out to 420 mm, and the magnification is now 11:1. The size of the subject will be about 2 mm. The numbers are similar to above but reversed. Image quality should be great. But depth of field will be very shallow, about 0.5 mm according to my calculations. And lighting the subject will be tricky. I'm guessing you will find it easier to use a microscope for this kind of application, but reversing the lens is a cheap and potentially good way to do it. Archibald |
#24
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Newbie question about macro with DSLR
On Mon, 28 Apr 2008 23:37:54 GMT, Archibald
wrote: On Sun, 27 Apr 2008 16:54:31 -0700, Eric Smith wrote: I'm considering buying a Canon Digital Rebel XSi for a project involving macro photography, and I'm looking for advice regarding macro lenses, reverse mounts, etc. Basically I'm trying to photograph a flat subject such that an area about 1 mm on a side will be full-frame. It would be better yet if I could do that for an area 0.5 mm on a side. The camera has an APS-C size sensor (22.2 x 14.8 mm). I have a precision X-Y stage with stepper motors and microstepping control, so I can position the subject in increments of less than 0.1 mm. I plan to take many photos with a small shift, and stitch them together, probably using Hugin. Because I expect there to be a lot of overlap between the images, I think I can tolerate some amount of vignetting, spherical abberation, etc. Is this feasible with some macro lens, combination of lenses, reversing ring, extension tube, etc? I'm hoping to avoid the need to buy a microscope and adapter, due to the cost, though that's obviously an option. I'm not sure how to tell whether a particular microscope would be suitable without trying it; for instance, I've looked at a 7x-90x microscope, but apparently 10x or 20x of that magnification is due to the magnification of the eyepiece, which I wouldn't have with the camera adapter. If I understand correctly, you need a magnification of around 10 to 20. By this I mean that the image size on the sensor of the camera is 10 to 20 times as big as what you are shooting. I agree with some other posters that your best solution might be a microscope. You should also be able to achieve this by reversing an ordinary Canon lens. A little background first... Lenses are designed to work best at particular subject and lens extension distances... normally the optimal subject distance is from infinity to around 10 times the focal length, and this corresponds to a lens to sensor distance of approximately the focal length plus a couple of millimeters. To use an example, consider a "normal lens", which would be about 35mm focal length for the Excess Eye (XSi). Depending in which lens exactly, it will work great focused from infinity to about 300 mm from the lens. If you try to get closer by using extension rings, you will be able to focus closer, but the optical quality starts to deteriorate a bit. When you get so close that you are near 1:1 (image and subject about the same size), image quality is worse, and continues to get worse as you go greater than 1. To some extent you can manage by stopping down, but that is of limited use. The fix is to reverse the lens when you need to shoot well over 1:1. Then the lens becomes happy again because its distances once again are close to its ideal distances... except that everything is in reverse. Thus the distance from the back of the lens to the subject is now about the same as lens-sensor when shooting at normal distances, and the distance from the front of the lens to the sensor is now around 300 mm or greater, which is about right for this lens. The light rays don't mind going backwards. To give some examples --- You are shooting a pair of shoes or whatever with the lens mounted normally. The distance to the subject is 400 mm. With that distance, the magnification on the sensor will be m = 0.1, and the lens extension is 3.4mm... so the distance from the center of the lens to the sensor is 35 + 3.4 = 38.4 mm. Now you reverse the lens using a reverse adapter, and shoot something very tiny. The lens-subject distance is very close at 38mm, the lens-sensor is racked out to 420 mm, and the magnification is now 11:1. The size of the subject will be about 2 mm. The numbers are similar to above but reversed. Image quality should be great. But depth of field will be very shallow, about 0.5 mm according to my calculations. And lighting the subject will be tricky. I'm guessing you will find it easier to use a microscope for this kind of application, but reversing the lens is a cheap and potentially good way to do it. Archibald Here's a link to a web site of someone who has adapted a bellows to a Canon for macrophotography -- check out the link at the bottom too. http://www.ganymeta.org/~darren/photo_bellows.php Archibald |
#25
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Newbie question about macro with DSLR
Eric Smith wrote:
I'm considering buying a Canon Digital Rebel XSi for a project involving macro photography, and I'm looking for advice regarding macro lenses, reverse mounts, etc. You are getting a lot of wonderful information... almost all of which is correct, but totally useless for your needs! Given what you have said you want to photograph, you CANNOT do it with typical "macro" techniques. Basically I'm trying to photograph a flat subject such that an area about 1 mm on a side will be full-frame. It would be better yet if I could do that for an area 0.5 mm on a side. The camera has an APS-C size sensor (22.2 x 14.8 mm). Okay, that means between 14.8:1 and 29.6:1 magnification. To put it simply: You can't do that with a macro lens on a DSLR. You need a microscope. You might also want to look at other types of cameras, because while it can be done with a DSLR that may not be the most reasonable in terms of either convenience or price. I have a precision X-Y stage with stepper motors and microstepping control, so I can position the subject in increments of less than 0.1 mm. I plan to take many photos with a small shift, and stitch them together, probably using Hugin. Because I expect there to be a lot of overlap between the images, I think I can tolerate some amount of vignetting, spherical abberation, etc. Do you want _each_ image to be even higher magnification than stated above, and for the resulting final product to be as described above???? Or do you mean each exposure will capture 0.5 to 1.0 mm of a larger object? If you mean that each exposure will capture 1mm of an object, then a typical dissecting microscope would be appropriate (and would be very convenient to mate with your X-Y state). Another possibility is a toolmakers metallurgical microscope. If the total object is 1mm, you need even greater magnification. In that case the metallurgical microscope is what you'll need. And even if you want 1mm portions of a larger object, you might want to look at this technique anyway, as it offers more flexibility than a dissecting scope. The feature you'll want to look for is the ability to mount it in a way that you can use your X-Y stage (which is not true of all metallurgical scopes). Another feature, which is almost universal though there is a chance you don't need it, is episcopic (or "epi" for short) illumination. That uses a beam splitter to illuminate the object through the same optical path that you view it. (That is probably _very_ useful to you.) Metallurgical microscopes also use special objective lenses that allow a greater lense to object distance. Microscopes can be very expensive, but it is unlikely that you need a fancy one. It should be fairly easy to find an older used model that will do fine. They often are available on eBay at reasonable prices, as are the parts and pieces such as objectives and "relay lenses" for photography. Keep in mind that objective lenses are matched to the eye pieces (and to the relay lenses used for projecting into a camera), and hence should not necessarily be mixed and matched between manufacturers. Is this feasible with some macro lens, combination of lenses, reversing ring, extension tube, etc? I'm hoping to avoid the need to buy a microscope and adapter, due to the cost, though that's obviously an option. I'm not sure how to tell whether a particular microscope would be suitable without trying it; for instance, I've looked at a 7x-90x microscope, but apparently 10x or 20x of that magnification is due to the magnification of the eyepiece, which I wouldn't have with the camera adapter. This is a *large* topic, which will take a good deal of research for you to get a handle on the perspective. Here is a set of tutorial articles on photography through a microscope: http://www.microscopyu.com/articles/...ing/index.html One thing to note is that due to the optical limitations of a microscope, the resolution of photographed images is limited. The practical effect is that high resolution cameras are *not* necessary, and might even be less useful! A very fancy DSLR is not going to get any better an image that some of the nicer, much less expensive, webcam's available! And adapting/using a web cam with a microscope may be much less expensive and easier to operate than mounting a DSLR on the same scope! In the end, the images are not different. -- Floyd L. Davidson http://www.apaflo.com/floyd_davidson Ukpeagvik (Barrow, Alaska) |
#27
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Newbie question about macro with DSLR
On Mon, 28 Apr 2008 19:41:18 -0800, (Floyd L.
Davidson) wrote: The feature you'll want to look for is the ability to mount it in a way that you can use your X-Y stage (which is not true of all metallurgical scopes). Another feature, which is almost universal though there is a chance you don't need it, is episcopic (or "epi" for short) illumination. That uses a beam splitter to illuminate the object through the same optical path that you view it. (That is probably _very_ useful to you.) Floyd, I was a dealer for Zeiss microscopes for 30 years. I've been to the Zeiss facilities in Germany. I've sold hundreds of microscopes and beam splitters. The beam splitter splits the optical path. It's used to provide a view for a second observer or for photography. The field of view is illuminated coaxially with the prime viewer completely independent of the beam splitter. The observer or camera is seeing the same field of view as the prime viewer, and that field is illuminated. The prefix "epi" means "above". There are two types of episcopes: one projects light from above for surface viewing, and the second - and the most common use of "episcope" - is a device that projects an image using a mirror. An overhead projector is an episcope. The kind most of us are familiar with, though, is: http://www.stds.fr/boutique/images_p...-AR225-481.jpg When we were kids, we used these to project an image - usually the comic pages - on a piece of paper taped to the wall - so we could trace over it. Microscopes can be very expensive, but it is unlikely that you need a fancy one. It should be fairly easy to find an older used model that will do fine. They often are available on eBay at reasonable prices, as are the parts and pieces such as objectives and "relay lenses" for photography. Keep in mind that objective lenses are matched to the eye pieces (and to the relay lenses used for projecting into a camera), and hence should not necessarily be mixed and matched between manufacturers. I can't figure out what you're saying here, and I'm pretty familiar with microscopes. I have no idea what "relay lenses" are. There are two ways to photograph through a microscope: (1) You attach a camera body to a port of the beam splitter. (2) You attach a camera body in place of an eyepiece. Some microscopes have built-in beam splitters with a single port, and some use a beam splitter that goes between the microscope body and the binoculars. I don't know what you mean by matching objective lenses to eyepieces. Naturally you have to have compatibility of manufacturer, but a microscope can be set up with several combinations of objective lenses and eyepieces. The combination determines the viewer's magnification and the field of view. I haven't been able to figure out, from the postings I've read, if he even really needs a camera body. There are systems available that send the image direct to a computer, and the computer captures the image. He might be able to work with an instrument like the digital boom microscope at http://www.microscope.com/omano-ezvu...ope-p-194.html because it doesn't appear that he needs to view the field through eyepieces at all. This would provide 7x to 45x magnification, or up to 90x with the Barlow lens. It could swing over his stage. I have no idea about the re-sale aspect of this type of instrument, but he could check with the company and see if an arrangement could be made. They may buy back the instrument at an agreed-on price if they can re-sell it. While $1,700 seems like it's over his budget, he'd might be spending $1,000 on a camera system with macro accessories that doesn't do what he wants done. -- Tony Cooper - Orlando, Florida |
#28
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Newbie question about macro with DSLR
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#29
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Newbie question about macro with DSLR
Eric Smith wrote:
If I buy an objective lens for a microscope, do you have any recommendations as to mounting it? How do I determine how far from the sensor it should be mounted? (Maybe I need to go back to school to study optics!) Mount it to a toolmaker's metallurgical microscope frame. he just need the objective and some sort of illumination system. Olympus makes, or at least used to make, the best equipment for this. I've looked at the Olympus site, and am somewhat lost. If it's not too much trouble, can you give me any more specific idea as to what I'm looking for? Olympus makes good equipment. So does Nikon. Oddly enough, for your purposes it will probably be best to look at some fairly old used equipment, including Unitron. The Olympus webpage has much good info, and these are a couple to start with: http://www.olympusmicro.com/primer/faq.html This one is long with lots of general information: http://www.olympusmicro.com/primer/microscopy.pdf Do read the whole thing, but first you might want to check these two sections: page 22, "Reflected Light Microscopy" page 37, "Digital Photomicrography" Here is another very useful URL which discusses selection of projection lenses for photomicography: http://krebsmicro.com/relayDSLR/relayoptics1.html This URL will help with general photomacrography information and with the terminology and has a chart of abbreviations: http://www.dyerlabs.com/microscopy/special.html -- Floyd L. Davidson http://www.apaflo.com/floyd_davidson Ukpeagvik (Barrow, Alaska) |
#30
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Newbie question about macro with DSLR
tony cooper wrote:
On Mon, 28 Apr 2008 19:41:18 -0800, (Floyd L. Davidson) wrote: For the OP, don't get sidetracked by Tony and I discussing all this techie crap at the beginning of this article. Go down to the end, if you didn't do so when you read Tony's article to start with, and check out the URL he provided for an example camera/microscope combination. It appears to be the perfect match! The feature you'll want to look for is the ability to mount it in a way that you can use your X-Y stage (which is not true of all metallurgical scopes). Another feature, which is almost universal though there is a chance you don't need it, is episcopic (or "epi" for short) illumination. That uses a beam splitter to illuminate the object through the same optical path that you view it. (That is probably _very_ useful to you.) Floyd, I was a dealer for Zeiss microscopes for 30 years. I've been to the Zeiss facilities in Germany. I've sold hundreds of microscopes and beam splitters. The beam splitter splits the optical path. It's Yes, that it does. Typically one path has a light attached, and the other has an eyepiece. That is how epi-illumination is typically provided. used to provide a view for a second observer or for photography. The That is, also, a not uncommon use for a beam splitter; but does not negate the fact that episcopic illumination is also done with a beam splitter. field of view is illuminated coaxially with the prime viewer completely independent of the beam splitter. The coaxial illumination is done with a beam splitter, that is entirely distinct from another beam splitter used to provide a camera port, or another beam splitter used to provide binocular viewing! There are beam splitters galore! The observer or camera is seeing the same field of view as the prime viewer, and that field is illuminated. The observer/camera is not looking at the illuminator though, which is the reason for using a beam splitter. viewing eye V | (beam splitter) |/ / aligning eye ====/ ==== light source / | | objective lense The light source shines into the beam splitter from the right side, and is split between one path going down (through the objective lense) to light the object being viewed and a path that goes straight through to the "aligning eye". Some designs actually have a port that can be visually observed to align the beam splitter and the light source, but most do not and just block that path. Light which reflects from the object being viewed shines upward into the beam splitter and is also divided into two paths. One goes straight upwards towards the "viewing eye" and the other shines into the light source port (where it is useless of course). The prefix "epi" means "above". There are two types of episcopes: The prefix "eip" means "A prefix, meaning upon, beside, among, on the outside, above, over. It becomes ep-before a vowel, as in epoch, and eph-before a Greek aspirate, as in ephemera" Note that "episcopes" is a different word than "episcopic". Regardless, epi-illumination is so called because the source of illumination is generally above the object being viewed, rather than below it as with transmitted light microscopy. (I see in a second post from you that this has been figured out. Do note that I did not choose the terminology, it standard terminology that the OP will need to be familiar with if he chooses to research this topic farther. Also note that while epi-illumination via the microscope is one method to provide reflected light illumination, it isn't the only one. Hence I said it is likely to be very useful, but might not be. With "dissecting" style microscopes it is more common to see ring lights and various forms of fiber optic illuminators.) If some particular light shading is necessary or useful to make the images show topographic detail better, it might not be epi-illumination that works best. one projects light from above for surface viewing, and the second - and the most common use of "episcope" - is a device that projects an image using a mirror. Do you see the connection with epi-illumination? An overhead projector is an episcope. The kind most of us are familiar with, though, is: http://www.stds.fr/boutique/images_p...-AR225-481.jpg When we were kids, we used these to project an image - usually the comic pages - on a piece of paper taped to the wall - so we could trace over it. Wonderful, but with no significance to the OP or too my discussion. Microscopes can be very expensive, but it is unlikely that you need a fancy one. It should be fairly easy to find an older used model that will do fine. They often are available on eBay at reasonable prices, as are the parts and pieces such as objectives and "relay lenses" for photography. Keep in mind that objective lenses are matched to the eye pieces (and to the relay lenses used for projecting into a camera), and hence should not necessarily be mixed and matched between manufacturers. I can't figure out what you're saying here, and I'm pretty familiar with microscopes. I have no idea what "relay lenses" are. That is an alternate term for "projection lense". Some might also call it a "camera lense" or a "photo lense", but those would be very rare. It replaces the ocular on a normal microscope when a camera is to be attached rather than a human observer. Here is a URL that I gave previously. It goes into detail. http://krebsmicro.com/relayDSLR/relayoptics1.html There are two ways to photograph through a microscope: (1) You attach a camera body to a port of the beam splitter. (2) You attach a camera body in place of an eyepiece. No, the camera body is not usually "in place of an eyepiece", though that can be done, it is rare. It is in _addition_ to *changing* the "eyepiece" (the ocular lense) to a projection (aka relay) lense. Otherwise the image will not likely "cover" the camera's sensor in a useful manner at the same point where the image is also in focus on the sensor. See the above URL. It's a complex subject. Some microscopes have built-in beam splitters with a single port, and some use a beam splitter that goes between the microscope body and the binoculars. I don't know what you mean by matching objective lenses to eyepieces. Naturally you have to have compatibility of manufacturer, but a microscope can be set up with several combinations of objective lenses and eyepieces. The combination determines the viewer's magnification and the field of view. There are at least two ways that oculars need to be matched to the objectives. One is due to dependency on the length of the tube and the other is to match compensation for optical aberration between the ocular and the objective lenses. Older microscopes were all based on a specific tube length, and the objective lenses are matched to that length (typically 160mm but sometimes a bit more or less). It can be a problem if an objective designed for a 160mm tube is used on a 210mm tube, for example. And modern microscopes are all designed for an "infinite" tube, which allows any length of tube to be used, but also requires that the objective and the ocular be matched. (The purpose is to allow addition of multiple optical devices into the optical path without changing the objective or ocular lenses. Again, they do need to be matched though, so only accessories from the same manufacture are likely to work together.) The second manner in which the objective and ocular lenses need to be matched is in regard to the compensation for optical aberration. Typically, oculars that have a 'C' or a 'K' in the designator are "compensating" oculars. For example, Zeiss KPL oculars have -1.4% compensation while Leitz uses -0.75% and Olympus used -0.7%. Nikon, near as I can tell, tended to use 0%, but I've never fully been able to confirm that. Whatever, it is clear that mixing oculars and objectives of those manufacturers will degrade the optics of a microscope. I haven't been able to figure out, from the postings I've read, if he even really needs a camera body. There are systems available that send the image direct to a computer, and the computer captures the image. He might be able to work with an instrument like the digital boom microscope at http://www.microscope.com/omano-ezvu...ope-p-194.html because it doesn't appear that he needs to view the field through eyepieces at all. This would provide 7x to 45x magnification, or up to 90x with the Barlow lens. It could swing over his stage. I have no idea about the re-sale aspect of this type of instrument, but he could check with the company and see if an arrangement could be made. They may buy back the instrument at an agreed-on price if they can re-sell it. While $1,700 seems like it's over his budget, he'd might be spending $1,000 on a camera system with macro accessories that doesn't do what he wants done. Bingo. You've hit what the OP needs dead on! At least, it sure looks like it. That assumes there is no other, unmentioned, need for a DSLR. I can't see how it can be done better for less money if new equipment is purchased. -- Floyd L. Davidson http://www.apaflo.com/floyd_davidson Ukpeagvik (Barrow, Alaska) |
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